FIGURE 2.
Selective orthosteric and an allosteric ligand for human FFA2 and FFA3 retain selectivity at the murine orthologs. Flp-InTM T-RExTM 293 cells harboring C-terminally eYFP-tagged forms of mFFA2 or mFFA3 were treated with Dox (500 ng/ml) to induce receptor expression. Membranes produced from these cells were used to measure [35S]GTPγS incorporation in response to various ligands shown previously to be selective for hFFA2 (A and C) or hFFA3 (B). The ago-allosteric behavior of 4-CMTB was preserved at murine FFA2 (D).