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. Author manuscript; available in PMC: 2012 Nov 30.
Published in final edited form as: Oncogene. 2010 Jun 14;29(31):4436–4448. doi: 10.1038/onc.2010.181

Figure 2. Expression of miR-661 is required for cell invasion and migration of invasive breast cancer cell lines.

Figure 2

(A), Transwell migration assay and Matrigel invasion assay (bottom) of MCF7-SNAI1 induced cells transfected with the miR-661-specific antisens LNA (LNA-661), or with the scrambled LNA (LNA-sc). (B), Real-time PCR analysis of the expression of epithelial markers (cytokeratin-18 (KRT18), E-cadherin, Claudin-3 and beta-catenin) in non-induced or induced-MCF7-SNAI1 cells, transfected with miR-661-specific antisense LNA (LNA-661), or with scrambled LNA (LNA-sc). (C), Real-time PCR analysis of miR-661 expression in six different cell lines. (D), Transwell migration assay and Matrigel invasion assay (bottom) of MDA-435 transfected with LNA-661 or with LNA-sc. Cell migration and invasion was analyzed 24 h after the seeding in the transwells. Results are represented as the mean +/−s.e.m (standard error of mean) of at least 3 independent experiments.