Figure 3. Effect of V-ATPase inhibitors on R1881-induced PSA secretion and cellular distribution.
(A) Baf.A treatment reduces PSA secretion. The LNCaP cells were incubated with 10 nM Baf.A in the presence and absence of 10 nM R1881 (24 or 48 hours) or with 0.01% DMSO (Control). The PSA secreted was quantified by ELISA and normalized to cell number. (B) Baf.A treatment disturbs PSA recruitment in the Golgi. The LNCaP cells treated as described in A were immunostained with antibodies to V1A and PSA and analyzed as described for Figure 2C. ***, p<0.001; **, p<0.01; significance levels relative to controls.