Fig 4.
HRV infection and propagation in primary nasal or bronchial epithelial cells. (A) HRV infection of undifferentiated primary nasal epithelial cells. Lysates of HRV-C15 (diamond) or HRV-A16 (open square) RNA-transfected HeLa cells were incubated with undifferentiated nasal epithelial cells, and HRV RNA replication was monitored by qRT-PCR at the indicated times. (B) Lysates of HRV-C15 or HRV-A16 RNA-transfected HeLa cells were incubated with differentiated nasal epithelial cells, and HRV RNA replication was measured as described for panel A. (C) Propagation of HRV-C15 in differentiated nasal epithelial cell cultures. Cell-free HRV-C15 viral particles were collected from an apical wash of infected differentiated nasal epithelial cells and used to infect separate naïve cultures of differentiated nasal epithelial cells as described in Materials and Methods. Viral RNA replication was measured by qRT-PCR at the indicated times after infection. (D) Infection of differentiated human bronchial epithelial cells. Lysates of HRV-C11 (closed circle)- or HRV-C15 (diamond)-transfected HeLa cells were incubated with differentiated bronchial epithelial cells, and HRV RNA replication was measured as described for panel A.