Fig 2.
Characterization of HRV-C15 viral particles produced after transfection of HeLa cells with HRV-C15 RNA. (A) Lysates of HRV-C15 RNA-transfected HeLa cells were subjected to potassium tartrate density gradient (10 to 40%) ultracentrifugation, and the presence of HRV-C15 VP1 protein in gradient fractions was detected by Western blot analysis using a polyclonal antibody directed against HRV-C VP1. Fraction numbers are indicated and are oriented from top to bottom of the gradient. Purified HRV-C VP1 (VP1) protein was included in the Western analysis for reference. M indicates the molecular size marker. (B) Electron microscopy (EM) images of sucrose cushion-enriched HRV-C15 viral particles or a mock control. Arrows indicate viral particles (triangle head) or empty particles (diamond head). For magnification of 110,000×, the scale bar is 100 nm; for magnification of 52,000×, the scale bar is 200 nm.