Figure 2. SUZ12 knockdown inhibits the growth of human EOC cells in vitro.
A, SKOV3 cells were infected with 2 individual lentivirus encoded shSUZ12 or control. 3 days post drug-selection, cells were examined for expression of SUZ12, H3K27Me3, H3K9Me3 and histone H3 by immunoblot. B, same as A, but examined for expression of H3K27Me3 and H3K9Me2 by immunofluorescence staining. DAPI counterstaining was used to visualize the cell nuclei. C, same as A, but equal number of drug-selected cells were seeded and counted at indicated time points using trypan blue exclusion assay that only counts for the viable cells. In addition, the cells were ensured to be in log phase throughout the experiments by splitting them before they reached confluence. Mean of 3 independent experiments with SD. * p=0.001 and ** p<0.001 compared with controls. D, same as A, 5000 drug-selected cells were seeded in soft agar and the number of colonies were counted after 3 weeks of culture. Mean of 3 independent experiments with SD. * p<0.001 compared with controls.