Figure 1.
(A) Effect of endothelial removal on APP expression in the aortas of wild-type mice. Please note that removal of the endothelium (E−) significantly reduced APP protein expression. eNOS expression is shown to control the experiments. Results are mean ± SEM (n = 4). *P < 0.05 vs. aortas with endothelium (E+). (B) Representative confocal images showing staining of thoracic aorta of wild-type mouse aorta with anti-APP (red) and anti-VE-cadherin (green) demonstrates colocalization of the two proteins mainly in vascular endothelium (yellow). 4′,6′-diamiddino-2-phenylindole dilactate (DAPI) was used to visualize nuclei. Data are representative of at least three independent experiments. L, lumen; A, adventitia. Bar denotes 50 µm.