Figure 2.
Ad5D24-CpG adenovirus displays increased NFκB activation compared with Ad5D24 while maintaining oncolytic efficacy in vitro. (a) Schematic representation of Ad5D24-CpG. This virus bears a 24 bp deletion in E1A gene for selective replication in cancer cells and 18 CpG-rich islands to increase Toll-like receptor 9 (TLR9) stimulation. (b) NFκB activation upon infection with Ad5D24 and Ad5D24-CpG at different MOI of viruses and different time points. P values; *P < 0.05, **P < 0.01, ***P < 0.001 (c) Oncolytic efficacy of Ad5D24-CpG by MTS cell-killing assay in different human tumor cell lines. MOI, multiplicity of infection; MTS, 3-(4,5,dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium; NF-κB, nuclear factor-κB; RLU, relative luciferase unit; VP, virus particle.