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Immunohistochemical analysis of Brachyury, E-cadherin, and vimentin in AdCC tissue. Sections of 4-μm thickness were used for the histopathological and immunohistochemical analysis as described in the Methods. A. Representative staining pattern of Brachyury on AdCC (a: tubular pattern, b: cribriform pattern, c: solid pattern). Bar = 50 μm.B. Serial AdCC sections were stained with HE (a) or immunostained with Brachyury (b), E-cadherin (c), and vimentin (d). Bar = 50 μm.
