Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Oct 24;8(9):1237–1247. doi: 10.7150/ijbs.4989

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

PMC Copyright notice

Fig 3 — Designer proteins for the study of IL-6 signaling. (A) Hyper-IL-6 is a fusion protein between the sIL-6R (blue) and IL-6 (brown). In a molecular model, the C-terminus of sIL-6R was 40 Å apart from the N-terminus of IL-6. Therefore, we inserted a flexible peptide linker between sIL-6R and IL-6 (black) to connect both molecules. (B) The sgp130Fc protein consists of the extracellular portion of gp130 linked to the Fc domain of a human IgG1 antibody. The sgp130Fc protein blocks IL-6 trans-signaling without affecting IL-6 classic signaling. (C) In the L-gp130 protein, the entire extracellular portion of gp130 is replaced by the leucine zipper of the Jun protein, leading to constitutive dimerization and activation of the gp130 protein. This protein can be used to study the effects of permanent gp130 activation in transfected cells or in tissue specific transgenic animals.