Figure 3.
Rapamycin promotes myelination by SCs. A, Schematic representation of the RM treatment paradigms. The black line indicates DIV, while the green lines represent the expression profile of myelin proteins in Wt (bold green) and neuropathic (dashed green) samples. The blue line indicates the time-scale for RM treatment in hours (h). Asterisks indicate the DIV at which the control samples Ct* and Ct** were obtained. For early RM treatment (a), starting on DIV9, or for late treatment (b) starting on DIV15, a pulse of RM (green arrows) was given for 48 h, followed by 48 h washout (wo, black arrows). This sequence was repeated and a third RM treatment (8 h) was given before analysis (arrowhead). B, C, DRG explants from Wt and C22 mice were treated with RM as indicated and whole protein lysates were analyzed for the myelin proteins, MBP and P0. The main isoforms of MBP are marked. Tubulin is shown as protein loading control. Molecular mass in kDa.