Skip to main content
. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Free Radic Biol Med. 2012 Aug 4;53(7):1440–1450. doi: 10.1016/j.freeradbiomed.2012.07.080

Figure 5. Inhibition of nitrite reduction to NO does not abolish nitrite-mediated biogenesis.

Figure 5

(A) Concentration of RSNO and Fe-NO generated in RASMC treated with nitrite (50μM) in the presence (open bars) or absence (filled bars) of Allopurinol (100μM) for 6 days. (B) Representative chemiluminescence trace of NO production by RASMC treated with nitrite (1mM) in the absence (black) or presence (gray) of Allopurinol (100μM). (C) Quantitation of NO production by RASMC treated with nitrite (1mM) and pre-treated with nothing (control), Allopurinol (100μM), Potassium ferricyanide (100 μM), or Potassium Cyanide (100 μM). (D) Mitochondrial to Nuclear ratio of RASMC treated with (open bars) or without (filled bars) nitrite (50 μM) in the presence of absence of Allopurinol (100μM). *p<0.01, #p<0.05. n>3.