Figure 2.
Histology of encapsulated cells by toluidine blue staining. (a) BHK-AK2 cells before grafting. Viable cells were located mainly adjacent to the capsule walls (left and right stripes), with the interior of the capsule also showing the collagen matrix. The square indicated is enlarged in b. (b) BHK-AK2 cells from a at higher magnification. (c) BHK-AK2 cells in capsule retrieved after having been grafted for 2 weeks in the lateral ventricle of control rats; an area adjacent to the capsule wall is shown. Note the dense clusters of viable cells. (d) BHK-AK2 cells in capsules kept in culture for 2 weeks. (e) BHK-AK2 cells retrieved from rat brain ventricles 24 days after grafting; scattered viable cells are surrounded by cellular debris. (f) BHK-WT control cells retrieved from rat brain ventricles 24 days after grafting. [Bars, 20 μm except in a (200 μm).]