Figure 2.
Histology of bone from 1α(OH)ase null mutant mice (−/−) and wild-type littermates (+/+). (A) Proximal tibial epiphysis, (GP) growth plate, and metaphysis at 4 weeks, hematoxylin and eosin. (Upper) Bar = 500 μm; (Lower) Bar = 250 μm. (B) Von Kossa stain of undemineralized sections at 4 weeks. Counterstaining with toluidine blue. (DF) distal femur, bar = 500 μm; (GP) growth plate, bar = 100 μm; (TB) trabecular and (CB) cortical bone, bar = 50 μm, *, osteoid. (C) Tartrate-resistant acid phosphatase (TRAP) staining of osteoclasts in the primary spongiosa. Counterstaining with methylene blue. (Upper) Bar = 250 μm; (Lower) Bar = 50 μm