Fig. 3.
Glp2r deletion in hypothalamic proopiomelanocortin (POMC) neurons. POMC-Glp2r KO mice were generated by crossing Glp2rfl/fl mice with POMC-Cre mice. Immunohistochemistry was performed on brain slices. Top: GLP-2R protein (in red) was colocalized in POMC neurons (in green, white arrows) in the ARC of POMC-eGFP, Glp2rfl/fl (WT) mouse hypothalamus. Bottom: efficiency of Glp2r deletion in POMC neurons was confirmed in POMC-Cre+/0, POMC-eGFP, and Glp2rfl/fl KO mouse hypothalamus. Note that GLP-2R protein (in red) is still expressed in non-POMC neurons (stars) but not expressed in POMC neurons (arrowheads), indicating that Glp2r was deleted specifically in POMC neurons.