Figure 6.
Cx36-mediated gap junctions shunt direct OSN signals on MCs. a, Example recordings (averages of 10–15 responses) from a MC in Cx36 KO mice (top) and WT mice (bottom). Note that, in KO mice, MCs have a large, rapid current absent in WT mice. b, Recording from an SMT cell from WT mice. c–e, Summary of EPSC measurements across 10 MCs in Cx36 KO mice, 11 MCs in WT mice, and 7 tufted cells (3 ET, 4 SMT cells) in WT mice. Plotted are onset delays (c), 10–90% rise times (d), and Amp4 ms values (e). f, OSNs directly drive spikes in MCs in Cx36 KO mice. fi shows four superimposed trials in MCs from KO mice (top) that included rapid-occurring spikes, absent in WT mice (bottom). The distribution of spike times in KO mice (fii) had a clear rapid component (open arrow). g, Cx36 KO did not significantly affect the amplitude of the OSN-EPSC in tufted cells, suggesting that OSN connectivity was not dramatically affected by KO. Error bars indicate SE.