Skip to main content
. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Gastroenterology. 2012 Jun 23;143(4):1006–1016.e4. doi: 10.1053/j.gastro.2012.06.034

Figure 4. Germ free mice have reduced colonic nitrergic neurons.

Figure 4

(A and B) Proximal colonic whole mount preparations stained for NADPH diaphorase and Acetylcholinesterase. (C–E) Distal ileum immunohistochemistry for the expression of nNOS, ChAT and TUJ1. The number of neurons stained for a specific marker was determined per unit area. At least 10 random fields were scored in a blinded fashion. Scale bar: 50 μm. Results are mean ± SE. n=6. *P < 0.05.