Figure 7.
A revertant suppresses the growth phenotype of the tom40 temperature-sensitive mutant KKY-Isp42–6. (A) Growth on agar plates of wild-type cells (Tom40wt), the Tom40 temperature-sensitive strain (Tom40ts) and a revertant (Tom40tssup). (B) The tom40 gene in the revertant was sequenced and one back mutation from Pro66 to the original wild-type Leu66 was identified. Two additional amino acid substitutions in the temperature-sensitive protein that are located in the vicinity of residue 66 are indicated.