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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Cell Microbiol. 2012 Feb 2;14(4):577–588. doi: 10.1111/j.1462-5822.2011.01743.x

Figure 2. Fluorescence analysis of cofilin and p-cofilin levels in P. gingivalis–infected HIGK cells.

Figure 2

A) P. gingivalis 33277-infected or ΔSerB-infected HIGK cells were labeled with antibodies to cofilin (upper panel) or p-cofilin (lower panel) and analyzed by CSLM. Controls were uninfected HIGKs. Magnification x63 of cells at 10 min time point. B) Fluorescence intensity analysis of p-cofilin:cofilin in infected or control HIGK cells. Intensity values were obtained using Leica Confocal software (arbitrary units) to calculate ratios. Results are representative of two independent experiments (n=3 coverslips/group). Data are means and error bars indicate standard deviation. **, P <0.01; ***, P <0.001 by Tukey-Kramer Multiple Comparison test.