Figure 6.
Inhibition of actin polymerization does not block migration in confinement. A) F-actin and α-tubulin after treatment with vehicle control, CD, or LA. MT protrusions formed in CD- and LA-treated cells. F-actin aggregates are also visible, but less prominent in LA-treated cells. Insets: ×2 views of boxed regions. B) Vehicle control cell migrating in a 3-μm channel. Intensity map depicts polarization of α-tubulin toward the cell front. C) Time-lapse image of a CD-treated cell entering a 3-μm channel (white arrowheads), but unable to exit (red arrowheads). Immunofluorescence analysis confirms F-actin disruption inside the channel and demonstrates the polarization of α-tubulin at the cell front in a CD-treated cell. D) LA-treated cell migrating through a 3-μm channel (red arrowhead indicates failure to spread and migrate on exiting the channel). Immunofluorescence confirmed the disruption of F-actin and polarity of α-tubulin.