Figure 2.
Hematological analysis of Ccnd3 knockout mice reveals a dramatic increase in erythrocyte size with a concomitant decrease in RBC number. (A–F) The hematological values from complete blood counts on mice of various genotypes are shown. The genotype +/+ indicates wild-type littermate controls, +/− indicates heterozygous animals, and −/− indicates Ccnd3 knockout animals. The data are shown as the mean, with the distribution of values for 11 +/+, three +/−, and 13 −/− animals, except for the reticulocyte percentage, which was done with five +/+, three +/−, and eight −/− animals. P-values are shown above the corresponding data and are based on a comparison with +/+ littermates. (G,H) Blood smears shown with identical (100×) magnification demonstrating the enlarged size and heterogeneity of erythrocytes from 8-wk-old Ccnd3−/− mice (H) in contrast to age-matched littermate +/+ controls (G). Bars: G,H, 5 μm.