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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Aging Cell. 2012 Jul 3;11(5):783–793. doi: 10.1111/j.1474-9726.2012.00842.x

Table 2.

Lifespan of sod1 RNAi flies and their controls fed rapamycin and N-acetyl cysteine.

Genotype Compound
conc.		 Sex Base diet Low calorie diet High sugar-low protein diet
# of
flies		 Mean±SE
(d)1, 2 		% Changes of
lifespan3 		# of
flies		 Mean±SE
(d)1, 2 		% Changes of
lifespan3 		# of
flies		 Mean±SE
(d)1, 2 		% Changes of
lifespan3
Rapamycin
UAS-sod1 IR/+ 0 µM ♂ 157 51.9±1.0 155 66.5±0.8 169 64.5±0.8
   (Wild type) 200 µM 148 55.0±1.5& 6.0% 145 58.7±1.4* −11.7% 180 63.0±1.1 −2.3%
0 µM ♀ 112 30.8±0.5 119 38.4±0.6 206 66.8±0.6
200 µM 115 38.8±1.2& 25.6% 137 60.1±1& 56.5% 206 61.3±1.1 −8.3%
sod1RNAi 0 µM ♂ 260 18.6±0.6 212 48.9±0.8 244 26.6±0.9
200 µM 238 14.6±0.3& −21.5% 196 23.8±0.6& −51.3% 282 19.6±0.9& −26.3%
0 µM ♀ 160 23.8±0.2 128 32.7±0.5 113 32.5±0.4
200 µM

NAC		 161 22.3±0.4 −6.4% 116 36.5±0.6& 11.9% 112 35.5±0.4& 9.4%
UAS-sod1 IR/+ 0 µg/ml ♂ 125 53.6±1.3 139 72.5±1.0 145 74.6±1.2
   (Wild type) 100 µg/ml 152 59.0±1.1& 10.1% 155 71.1±0.9 −1.9% 149 74.4±1.0 −0.3%
1 mg/ml 138 56.4±1.1 5.2% 132 70.9±1.0 −2.2% 128 68.7±1.0& −7.9%
0 µg/ml ♀ 166 33.2±0.7 162 43.7±0.9 149 68.6±0.8
100 µg/ml 159 31.0±0.5# −6.60% 157 42.5±0.9 −2.7% 152 67.4±0.9 −1.7%
1 mg/ml 152 33.0±0.7 −0.60% 159 38.6±0.9& −11.7% 152 68.2±0.8 −0.6%
sod1RNAi 0 µg/ml ♂ 147 21.8±0.5 155 53.2±1.2 150 27.2±0.7
100 µg/ml 146 22.0±0.6 0.9% 146 53.1±0.8 −0.2% 111 28.5±1.0 4.8%
1 mg/ml 140 20.8±0.5 −4.6% 147 44.2±1.0 −16.9% 129 31.2±1.1# 14.7%
0 µg/ml ♀ 156 24.6±0.3 163 36.5±0.6 150 46.0±0.8
100 µg/ml 161 26.0±0.3 5.70% 159 35.5±0.6 −2.7% 156 40.0±0.7& −13.0%
1 mg/ml 164 24.4±0.3 −0.80% 153 29.9±0.6& −18.1% 156 36.4±0.6& −20.9%
1

Lifespan is expressed as mean±Standard error (SE) in days (d).

2

Significant changes are marked with superscripted symbols. P values were based on comparing flies fed the rapamycin or N-acetylcysteine (NAC) supplemented diet to genotype- SY diet- and gender-matched non-supplemented controls by logrank test.

*

p <0.05;

#

p <0.01;

&

p <0.001.

3

Percentage changes were relative to diet-, gender- and genotype matched non-supplemented controls.