Figure 4. Pam3Cys and FSL-1 induce surface adhesion molecule and cytokine expression in human lung microvasculature endothelial cells (HMVEC-L).
HMVEC-L monolayers were treated with Pam3Cys or FSL-1 (10 µg/ml) for 20 h (n = 4). One-way ANOVA with Dunnett’s multiple comparison post test (media vs. TLR2 agonists): **p < 0.01. (A–D) Levels of IL-6, IL-8, GM-CSF and G-CSF were quantified using sandwich ELISAs on HMVEC-L culture supernatants. (E and F) Surface expression of adhesion molecules ICAM-1 and E-selectin was assessed using a cell-based ELISA.
