Figure 6 .
Dynamic responses of subpopulations and metabolites in glucose gradient experiments mimicking large-scale cultivation conditions. An aerobic glucose-limited chemostat was perturbed with a low concentration glucose pulse. Cells were sampled from the bioreactor and exposed to freeze-thaw stress and subsequently analysed with flow cytometry. Symbols: Subpopulation P1 (high GFP level, intact cell membranes) (crosses); Subpopulation P2 (low GFP level, permeabilised cell membranes) (snowflakes); Glucose (open circles); Acetate (open diamonds); Ethanol (open squares); Glycerol (open triangles).