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. Author manuscript; available in PMC: 2013 Aug 24.
Published in final edited form as: Immunity. 2012 Aug 24;37(2):209–222. doi: 10.1016/j.immuni.2012.05.027

Figure 4. STAT3 promotes the loss of FoxP3 expression in nTreg cells during acute-GvHD.

Figure 4

Irradiated allogeneic (BALB/c) mice were transplanted with CD45.1+ B6 T-depleted BM cells together with 0.5 × 106 CD45.2+CD4+FoxP3-GFP+ T cells from either Foxp3-GFP;Stat3fl/fl (Control) or Foxp3-GFP;CD4-Cre;Stat3fl/fl mice (Stat3KO). FoxP3 expression was determined in the CD4+ CD45.2+ CD45.1−H2kb+ population at day 14 post BMT. Flow cytometry plots show a representative sample; the histogram shows mean values from three mice pooled from two separate experiments (A). Irradiated syngeneic (B6) or allogeneic (BALB/c) mice were injected with CD45.1+B6 T-depleted BM cells together with 2x 106 CD45.1+ CD25− wild-type B6 T cells and 0.5 × 106 CD45.2+ CD4+ FoxP3-GFP+ T cells from Foxp3-GFP;Stat3fl/fl mice. FoxP3 expression was determined in the CD4+ CD45.2+ CD45.1− population at day 10 post BMT. Flow cytometry plots show a representative sample; the histogram represents mean values from three mice each derived from an independent experiment. Significance was determined by a two-tailed paired t test (B). Irradiated allogeneic (BALB/c) mice were injected with CD45.1+ B6 T-depleted BM cells together with 2 × 106 CD45.1+ CD25− wild-type B6 T cells and 0.5 × 106 CD45.2+ CD4+ FoxP3-GFP+ T cells from either Foxp3-GFP;Stat3fl/fl (Control) or Foxp3-GFP;CD4-Cre;Stat3fl/fl (Stat3KO) mice. FoxP3 expression was determined in the CD4+ CD45.2+ CD45.1− H2Kb+ population at day 10 post-BMT. The flow cytometry plots show a representative sample; the histogram indicates mean values pooled from three mice derived from two separate experiments (C).