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Fluorescence photoswitching of (A) Cy3/5DNA, (B) Cy*DNA, and (C) Cy5DNA. The sample was immobilized onto a polylysine-coated coverglass and imaged in a chamber containing switching buffer. The molecules were continuously excited at 633 nm, and pulses of 532 nm were used to photoactivate fluorescence (represented by the dashed lines).
