Figure 4.
TRAIL enhances the oncolytic effect of tumor-specific, RC Ad in cancer cell lines. (a) Hep3B and (b) DLD-1 cells were infected with KD3, TetON–TRAIL+KD3, TetON–EGFP+KD3, KD3–IFN, TetON–TRAIL+KD3–IFN or TetON–EGFP+KD3–IFN at an MOI of 10PFU per cell for each virus in the presence or absence of Dox. Cell viability was quantified at day 3 (Hep3B) or day 2 (DLD-1) postinfection. EGFP, enhanced green fluorescent protein; IFN, interferon; MOI, multiplicity of infection; PFU, plaque forming unit; RC, replication competent; TRAIL, tumor necrosis factor-related apoptosis-inducing ligand.