Skip to main content
. Author manuscript; available in PMC: 2012 Sep 4.
Published in final edited form as: Cell. 2011 May 13;145(4):584–595. doi: 10.1016/j.cell.2011.03.050

Figure 4. HSD17B14 mediates ADIOL generation in BV2 microglia cells and regulates inflammatory responses.

Figure 4

A. A partial scheme of the biosynthesis and metabolism of ADIOL. B. HSD17B14 mediates conversion of DHEA to ADIOL. COS-1 cells were transfected with the indicated expression vectors, using HSD17B1 as a positive control, and the products of DHEA conversion were monitored by gas chromatography at 0, 1 and 6 hr. Retention times for DHEA and ADIOL standards are indicated. C. Knockdown of HSD17B14 in BV2 cells by stable transduction with a lentiviral vector directing expression of a specific shRNA inhibits conversion of DHEA to ADIOL. ADIOL levels in media were quantified 24 hr after addition of DHEA. D. Stable knockdown of HSD17B14 using two different shRNAs blocks the ability of DHEA, but not ADIOL or Indazole-Cl, to suppress LPS induction of IL-6 mRNA in BV2 cells. Cells were pre-treated with the indicated ligands for 1 hour except for DHEA, which was added 6 hours prior to LPS stimulation. *p < 0.01 compared to EtOH treated and LPS stimulated sample. Error bars represent SD. See also Figure S4.