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. 2012 Sep 4;7(9):e44667. doi: 10.1371/journal.pone.0044667

Figure 2. Ca2+ handling and oxidative phosphorylation capacity of subsarcolemmal (SSM) and interfibrillar (IFM) mitochondria.

Figure 2

A, Typical tracing of Ca2+ loading in SSM (left panel) and IFM (right panel). Mitochondria were loaded with consecutive Ca2+ pulses (arrows), and each pulse delivers 50 nmol of Ca2+. B, Average Ca2+-accumulating capacity of SSM (open bar) and IFM (filled bar), n = 6, p<0.05. C, Typical tracing of ADP-induced changes in oxygen consumption (top) and changes in membrane potential from baseline (bottom) of SSM and IFM. D, Average ADP-stimulated (State 3) respiration of SSM and IFM (n = 6, p<0.05). E, The rate of ATP production in SSM (left bars) and IFM (right bars) before (B/W bars) and after (gray bars) loading with 150 nmol Ca2+/mg protein, n = 3, p<0.05. Inset: Alternative monitoring of ATP production in mitochondria using coupled enzymes. Asterisks signify statistical differences with p<0.05.