Figure 2.
Drosulfakinin deficient flies consume more food than controls. Ingested indigo dyed food was visually detected in the abdomen of transgene flies. In all experiments in this and other figures parental controls were crossed to wildtype w1118 flies (reported as, e.g., Dilp2/+). After 15 min of feeding, an average of 98.6% of the flies with Dsk-knockdown in all DSK-expressing cells, DPCs (Dsk/Dsk-RNAi) and 99% of the flies with Dsk-RNAi in IPCs (Dilp2/Dsk-RNAi) had fed on blue food, compared to only 64.5 and 66.7% of respective controls, Dilp2/+, Dsk/+, and Dsk-RNAi/+ (p < 0.001, n = 90–112 for the different genotypes, One-way ANOVA). All experiments were performed in three replicates, ***p < 0.001.