Skip to main content
. Author manuscript; available in PMC: 2013 Mar 23.
Published in final edited form as: Immunity. 2012 Mar 15;36(3):348–361. doi: 10.1016/j.immuni.2012.02.010

Figure 2. Deletion of E-proteins on a TCR transgenic background generates CD8+ T cells expressing components of a MHC II-restricted TCR.

Figure 2

Tcf12f/fTcfe2af/fCd4cre+ mice were crossed onto an OT-II TCR transgenic background. (A) Representative staining of thymus and spleen from indicated genotypes. Percentages in each quadrant are displayed. Percent means ± SD for the CD4 SP (CD4+CD8−) thymocyte gate were 11 ± 0.74 for control (n=5); 28 ± 2.1 for OT-II+ (n=5); 3.3 ± 1.8 for cre+ (n=4); 5.2 ± 2.1 for cre+OT-II+ (n=7). Percent means ± SD for the splenocyte CD4+ T (CD4+CD8−) and CD8+ T (CD4−CD8+) gates, respectively, were 18 ± 5.7 (n=5) and 8.7 ± 2.3 (n=7) for control; 17 ± 4.6 (n=4) and 2.2 ± 0.63 (n=5) for OT-II+; 4.8 ± 0.92 (n=4) and 29 ± 9.2 (n=5) for cre+; 7.6 ± 2.2 (n=6) and 21 ± 11 (n=8) for cre+OT-II+. (B) Representative staining of spleen from indicated genotypes. Plots are pre-gated on CD4+ or CD8+ cells, as labeled. Percentages in each quadrant are displayed. Percent means ± SD for the splenocyte Vα2+Vβ5+ gate within the CD8+ T cell compartment were 1.4 ± 0.32 (n=5) for control; 14 ± 5.2 (n=3) for OT-II+; 0.20 ± 0.044 (n=3) for cre+; 35 ± 17 (n=6) for cre+OT-II+. See also Figure S2.