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Western blot analyses of E-cadherin and Snail in SNU719 cells treated with both PGE2 and IL-1β. There is no synergic effect of IL-1β and PGE2 on the expression pattern of E-cadherin and Snail. Twenty µg of protein was separated by 10% SDS-polyacrylamide gel electrophoresis and transferred to a nitrocellulose membrane. The bottom represents GAPDH, which was used as a loading control.
