Figure 2. Detection of a punctate LC3-staining pattern in murine wild-type pancreas and uniform staining in atg7−/− tissue. Immunohistochemical staining for ATG7, LC3, SQSTM1/p62 and ubiquitin in murine wild-type pancreata (top two rows) and in consecutive sections from pancreata with mosaic deletion of ATG7 (atg7−/−, bottom two rows). Panels with straight borders are complete, i. e. not cropped and representative overview images taken at 40x magnification with a scale bar (straight) that represents 100 µm. Panels with dashed borders are zoomed and cropped sections from the overview panels (cropped regions are indicated by the small dashed rectangle in the overview panels). The dashed scale bars in the cropped panels represent 20 µm. IHC was done on consecutive sections for the atg7−/− samples and each display two large, not recombined (i.e., Atg7wt/wt) regions of pancreatic acinar tissue that are marked as (*, **) and encircled in red. In addition two pancreatic islets (#, ##) are also marked and encircled which act as landmark structures to enable clear alignment of sequential sections. Antigen retrieval for LC3 was undertaken in Tris-EDTA, pH 9 for LC3 and in 10 mM Na Citrate buffer, pH 6 for ATG7, SQSTM1/p62 and ubiquitin.