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Time plot of association of [3H]ZM 241385 to HEK-293 cell membranes expressing the human A2B receptor. Total binding (○). non-specific binding (▲), and specific binding (●) are shown. [3H]ZM 241385 (30 nM) was incubated wilh 18 μg of membranes at 25 °C. Non-specific binding was determined with 20 μM CPX.
