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. Author manuscript; available in PMC: 2012 Aug 20.
Published in final edited form as: Nature. 2011 Mar 31;471(7340):629–632. doi: 10.1038/nature09863

Figure 2. MHC I–peptide transfer does not require exosomes but does require cell contact between donor and recipient cells.

Figure 2

a, Exosomes recovered from the supernatants of peptide-pulsed B6 or H–2Kbm1 dendritic cells were incubated alone (exosomes alone) or with B6 dendritic cells or H–2Kbm1 dendritic cells and cultured with CFSE-labelled OT-I cells for 60 h. Representative flow cytometry profiles are shown. b, Dendritic cells labelled with either CMFDA (cytoplasmic dye (green)) or Dil (membrane dye (red)) were cultured together. A representative frame is shown. c, d, B6.SJL peptide-pulsed dendritic cells were cultured with unloaded H–2Kbm1 dendritic cells mixed together (c; −transwell) or separated by a transwell (d; +transwell). Dendritic cells were then separated by cell sorting and cultured with CFSE-labelled OT-I cells for 60 h. Representative flow cytometry profiles are shown. Numbers above the plots indicate the number of dendritic cells cultured per well.