Fig 5.
DSS compounds do not inhibit HBV polymerase activity. HBV virion particles purified from HepDE19 culture fluid were subjected to endogenous polymerase reaction with [32P]dCTP plus CCC-0975 or CCC-0346 at the indicated concentrations. DMSO at 0.1% was used as a solvent control, and ddGTP was a reference chain terminator in the reaction. Incorporated [32P]dCTP radioactivity was measured with a liquid scintillation counter. The relative polymerase activity was plotted as a percentage of the readout of counts per minute in the control reaction (y axis). Values are averages of triplicate samples, and error bars represent standard deviations.