Figure 4.
Subcellular localization of the SCRIB protein interaction network. (a–d) Confocal images of MDA-MB-231 cells stained with the following antibodies to detect endogenous SCRIB-associated proteins: (a) SCRIB and NOS1AP, (b) SCRIB and ARHGEF7, (c) SCRIB and VANGL, or (d) NOS1AP and VANGL. Signals from individual antibodies are shown in the left and center columns, whereas a pseudo-colored merged image is included in the right column, with 4,6-diamidino-2-phenylindole (DAPI) DNA stain shown in blue. Areas of colocalization (yellow color) are indicated by white arrows. In all images scale bars are 30 μm.