Table A1.
Primers used in this work.
| Primer | Sequence* | Description |
|---|---|---|
| GBRORF0459 DN-F | TCCCCCGGGAAAATAATTGACCATGGCΔ | ΔBAB1_0061 |
| GBRORF0459 DN-R | AACTGCAGGGGCAGGCCCGCCGCCT | |
| GBRORF0459 UP-F | GTGCACCGCATTTATTGCGT | |
| GBRORF0459 UP-R | TCCCCCGGGTTGCAGATCAAAGAGCG | |
| GBRORF0206 DN-F | TCCCCCGGGAGCCTGCGTTATGCTGCCCGG | ΔBAB1_0355 |
| GBRORF0206 DN-R | AAACTGCAGCACCACGAAAATGGCCGTG | |
| GBRORF0206 UP-F | GCAGGCGCTCAAAATAATGTG | |
| GBRORF0206 UP-R | TCCCCCGGGCTTTGCGGGGGGCGTTCA | |
| GBRORF0049 DN-F | TCCCCCGGGATAGGTGTGCGCCCCGC | ΔBAB1_0513 |
| GBRORF0049 DN-R | AACTGCAGAAGTCGAATCCGCCCTC | |
| GBRORF0049 UP-F | CCGGCCTTCATGAGCCGGAT | |
| GBRORF0049 UP-R | TCCCCCGGGTCGTTTTGCGGGCCAAC | |
| GBRORF2128 DN-F | TCCCCCGGGCCGTTGTTATAAACGACGAC | ΔBAB1_0581 |
| GBRORF2128 DN-R | AAACTGCAGAGCTTTTACCAAAGAACAGG | |
| GBRORF2128 UP-F | GGCTCATACCCGCCAAATGCA | |
| GBRORF2128 UP-R | TCCCCCGGGTTCAGCCGTTCCAGCACTT | |
| GBRORF2083 DN-F | TCCCCCGGGAGAAGAAACTGTTGTT | ΔBAB1_0626 |
| GBRORF2083 DN-R | AAACTGCAGGTCCTTGTCGTCTTCAACA | |
| GBRORF2083 UP-F | CTGCTCAGATCGCCGGATGGC | |
| GBRORF2083 UP-R | TCCCCCGGGATTTATTGGCTGTCATATTGTT | |
| GBRORF1960 DN-F | TCCCCCGGGACGACCTGCTTTCGCAGAT | ΔBAB1_0743 |
| GBRORF1960 DN-R | AAACTGCAGCCCGTATGTAACAATGAG | |
| GBRORF1960 UP-F | CCTATGCAGCTTCCACCATTG | |
| GBRORF1960 UP-R | TCCCCCGGGTAAGTTTGTGTGCTCATTC | |
| GBRORF1747 DN-F | TCCCCCGGGCTATCCCGCGACGAGCG | ΔBAB1_0960 |
| GBRORF1747 DN-R | AAACTGCAGAAGCTGATCTTCATCGAT | |
| GBRORF1747 UP-F | GATAGTGCCAGGATAAGGAAA | |
| GBRORF1747 UP-R | TCCCCCGGGATGGAAAACTTCATCATT | |
| GBRORF1716 DN-F | TCCCCCGGGTCAAGAAATTCAACCTT | ΔBAB1_0992 |
| GBRORF1716 DN-R | AACTGCAGATCGCCTGATGGCAATC | |
| GBRORF1716 UP-F | TCCGATTGGGCGTAGCTTAC | |
| GBRORF1716 UP-R | TCCCCCGGGTACTGGCGCTGGCGTCT | |
| GBRORF1608 DN-F | TCCCCCGGGACGCTCATCACCTTGACGGA | ΔBAB1_1106 |
| GBRORF1608 DN-R | AAACTGCAGCCCTCCCGCCCCTCACGT | |
| GBRORF1608 UP-F | GGCGAGAGGGATGAAGATCGA | |
| GBRORF1608 UP-R | TCCCCCGGGTCCATGAAGTTGAAGATTT | |
| GBRORF1520 DN-F | TCCCCCGGGCGATTCCATTAAAACAG | ΔBAB1_1194 |
| GBRORF1520 DN-R | AACTGCAGAATCATGGCTCTAAATC | |
| GBRORF1520 UP-F | CCTTGGCATGGCGCTGCGTC | |
| GBRORF1520 UP-R | TCCCCCGGGCCTGTCCTTCGCTCGGG | |
| GBRORF1512 DN-F | TCCCCCGGGCGTTGCCGTCGGCCATAG | ΔBAB1_1204 |
| GBRORF1512 DN-R | AACTGCAGCCTGCGGTCCATTCGTA | |
| GBRORF1512 UP-F | GTTGTGCTACTTCATTTACG | |
| GBRORF1512 UP-R | TCCCCCGGGTCGCCGCACCGCCTG | |
| GBRORF1452 DN-F | TCCCCCGGGCGACGACGAAGAGGAAGAG | ΔBAB1_1262 |
| GBRORF1452 DN-R | AAACTGCAGACGCCTTCGTTCATCT | |
| GBRORF1452 UP-F | CAGTGCAGGAACCTTATTGCG | |
| GBRORF1452 UP-R | TCCCCCGGGACTCGGATGTGCTTCAATAGC | |
| GBRORF1325 DN-F | TCCCCCGGGTTTGCCGCATGGCTCGACGC | ΔBAB1_1390 |
| GBRORF1325 DN-R | AAACTGCAGCGACGGAGTGGCGGCTCT | |
| GBRORF1325 UP-F | GGCCAGATAGCCCGGAACGA | |
| GBRORF1325 UP-R | TCCCCCGGGACCCACATAACATCTAAAGGA | |
| GBRORF1323 DN-F | TCCCCCGGGCATCAGGCGGAGTGACC | ΔBAB1_1392 |
| GBRORF1323 DN-R | AAACTGCAGGACGTCGGCCTCCATTCTC | |
| GBRORF1323 UP-F | GCTTGTGACGCTGGAGGAAAA | |
| GBRORF1323 UP-R | TCCCCCGGGTCCGACATTTCCGTTATCAAC | |
| GBRORF0969 DN-F | ACCCCGGGCTTTCACCTGCACGAAG | ΔBAB1_1743 |
| GBRORF0969 DN-R | ACCTGCAGACCTATGGCGTGGATTCG | |
| GBRORF0969 UP-F | ACGCGCGATGGCTCAATGC | |
| GBRORF0969 UP-R | ACCCCGGGCAGGATTTTGGTCAC | |
| GBRORF0964 DN-F | TCCCCCGGGTATCCCCATCGAGCTTGCC | ΔBAB1_1748 |
| GBRORF0964 DN-R | AAACTGCAGGATCGCCCGAATGCGATT | |
| GBRORF0964 UP-F | CCGACACCCAGATTCATGCGC | |
| GBRORF0964 UP-R | TCCCCCGGGTTCTTGAAAACGTCTTTCATG | |
| GBRORF0765 DN-F | TCCCCCGGGTTGATGTCCTTCAATTC | ΔBAB1_1939 |
| GBRORF0765 DN-R | AACTGCAGGATGCGCCAGACGAA | |
| GBRORF0765 UP-F | ACGGCGCATGCTTCTTGTCG | |
| GBRORF0765 UP-R | TCCCCCGGGGGGCTGGTTCGGACAAT | |
| GBRORF0663 DN-F | TCCCCCGGGCAATACGTTCCGGCGAAG | ΔBAB1_2041 |
| GBRORF0663 DN-R | AACTGCAGGTGTCGAGAAGCTCAAG | |
| GBRORF0663 UP-F | CCTGCATCTGCAAAGGCC | |
| GBRORF0663 UP-R | TCCCCCGGGTGGTTGATGCCATCATT | |
| GBRORFA0247 DN-F | TCCCCCGGGTGTGCGCGGCCAAGATAA | ΔBAB2_0160 |
| GBRORFA0247 DN-R | AAACTGCAGCCCTGCAAAATTTCCTGT | |
| GBRORFA0247 UP-F | CCATGGGTATGATGATGCTGC | |
| GBRORFA0247 UP-R | TCCCCCGGGTTTCCCGCAAGCTGCAAGC | |
| GBRORFA0258 DN-F | TCCCCCGGGCGCTTGCCGCCGCCTGATT | ΔBAB2_0170 |
| GBRORFA0258 DN-R | AAACTGCAGATTGGTGAGGGGGCGAAA | |
| GBRORFA0258 UP-F | CCGCTTCGTCGAACAGGCAAC | |
| GBRORFA0258 UP-R | TCCCCCGGGGCGTATTCGACATTCTAC | |
| GBRORFA1086 DN-F | TCCCCCGGGCTTTCCATCGCCTGAAACTGT | ΔBAB2_0232 |
| GBRORFA1086 DN-R | AAACTGCAGGGGAAATGTAGGAGGTAG | |
| GBRORFA1086 UP-F | GAAGAAGGCCTTATCTGATCA | |
| GBRORFA1086 UP-R | TCCCCCGGGCCGCCCCCTCTTCACCATGA | |
| GBRORFA0924 DN-F | TCCCCCGGGTGGAACACTCTCCCTGC | ΔBAB2_0400 |
| GBRORFA0924 DN-R | AACTGCAGTCCTTTGCTGCCTTTGT | |
| GBRORFA0924 UP-F | GCGTATCAGCCATGCAGGCG | |
| GBRORFA0924 UP-R | TCCCCCGGGTAAGACGTAGGTTAGGC | |
| GBRORFA0907 DN-F | TCCCCCGGGCAGCAATGGTTCGACGAAAG | ΔBAB2_0415 |
| GBRORFA0907 DN-R | AAACTGCAGACAAATGCACCGAGGAT | |
| GBRORFA0907 UP-F | GCGGATCGGCGAGACTTTTCC | |
| GBRORFA0907 UP-R | TCCCCCGGGCAGTTCCATGACCCGTTCT | |
| GBRORFA0873 DN-F | TCCCCCGGGCGCTTCCCGCCTACTACTGA | ΔBAB2_0450 |
| GBRORFA0873 DN-R | AAACTGCAGCCATAATGTCCGCAAAC | |
| GBRORFA0873 UP-F | GGTTTTGCAGTGGCTTCCAG | |
| GBRORFA0873 UP-R | TCCCCCGGGGGCAGCAAACTGGACATT | |
| GBRORFA0854 DN-F | TCCCCCGGGCTTCTCATCCGCAAGCTA | ΔBAB2_0470 |
| GBRORFA0854 DN-R | AAACTGCAGATAGCGCGGCTTGTGCAC | |
| GBRORFA0854 UP-F | CATGTTCAAGCGCACGATCCG | |
| GBRORFA0854 UP-R | TCCCCCGGGATATGAATTGATTGACATCAC | |
| GBRORFA0598 DN-F | TCCCCCGGGCAACAAGCACCACTGAAATG | ΔBAB2_0726 |
| GBRORFA0598 DN-R | AAACTGCAGATATGGCCCGGCGCGGCAT | |
| GBRORFA0598 UP-F | CGGCGGCTTCGTGCCGGCGC | |
| GBRORFA0598 UP-R | TCCCCCGGGTTGCTCTCATCCTCGATCA | |
| GBRORFA0563 DN-F | TCCCCCGGGCCTATGCAGAAGCCCTG | ΔBAB2_0761 |
| GBRORFA0563 DN-R | AACTGCAGAAGAGAATATGGCCGTC | |
| GBRORFA0563 UP-F | CGCTTTTTCCCGGCAGCATG | |
| GBRORFA0563 UP-R | TCCCCCGGGTTCGGTTTCAGTTTCTC | |
| GBRORFA0429 DN-F | TCCCCCGGGCTGAAGCAGAACTCAAGAAAGTT | ΔBAB2_0862 |
| GBRORFA0429 DN-R | AAACTGCAGTTTAATGAGATATTTC | |
| GBRORFA0429 UP-F | CAATGCAGGCGCAATTGCAAC | |
| GBRORFA0429 UP-R | TCCCCCGGGAAGAGAGCCTTAATCATTTCC | |
| GBRORFA1199 DN-F | TCCCCCGGGTTCCCGTTTCTGCGACG | ΔBAB2_1072 |
| GBRORFA1199 DN-R | AAACTGCAGATAACGGCATCAGCACCG | |
| GBRORFA1199 UP-F | GGGCACTTCTTCTGCCACGT | |
| GBRORFA1199 UP-R | TCCCCCGGGATAGTTTCGGCTTCGAT | |
| GBRORFA0014 DN-F | TCCCCCGGGATGAGTCGCAAAAGCGGAG | ΔBAB2_1087 |
| GBRORFA0014 DN-R | AAACTGCAGGGTTGCCGGCGCCTCTG | |
| GBRORFA0014 UP-F | GGCCGCCGTGCTGGCGTTTTG | |
| GBRORFA0014 UP-R | TCCCCCGGGCGTGTTCCAGCCTGCGTCAT | |
| GBRORFA0062 DN-F | TCCCCCGGGTAGGCTTATCCAGGCTGTA | ΔBAB2_1136 |
| GBRORFA0062 DN-R | AAACTGCAGGCCTATGGTCTCTTCCCGG | |
| GBRORFA0062 UP-F | CCGCTTCTCACATCCATTTTC | |
| GBRORFA0062 UP-R | TCCCCCGGGCGATGCTGTTATCTTCGAT | |
| GroEL-P-F | AGCATGCCGTTCGGGCGATGGAAACAGGCGTG | Promoter PGroEL |
| GroEL-P-R | ACATATGGTATAACCCTTGGTGTTATAGACG | |
| dTomato-F | ACATATGGTGAGCAAGGGCGAGGAGGTC | pGroEL/dsRed |
| dTomato-R | ACCCGGGATGCATTACTTGTACAGCTCGTCCATGCCGTAC | |
| A0598-Flag-F | ACATATGAGAGCAACCACGCTTAC | pGroEL/CydX |
| A0598 -PstI | ACTGCAGTCAGTGGTGCTTGTTGCCTTC | |
| CydB-F-NdeI | ACATATGATACTCAGCGATTTGTTGGACTATC | pGroEL/CydB |
| CydB-R-PstI | ACTGCAGTCAGTAAGCGTGGTTGCTCTCATCCTC | |
| CydB-F-NdeI | ACATATGATACTCAGCGATTTGTTGGACTATC | pGroEL/CydBX |
| A0598 -PstI | ACTGCAGTCAGTGGTGCTTGTTGCCTTC | |
| A0598-Flag-F | ACATATGAGAGCAACCACGCTTAC | pCydX/FT |
| A0598-Flag-R | AGTCGACGTGGTGCTTGTTGCCTTC | |
| A0598 -XbaI | ATCTAGAAGAGCAACCACGCTTACTGATTG | pFT/CydX |
| A0598 -PstI | ACTGCAGTCAGTGGTGCTTGTTGCCTTC | |
| Promoter-F | TTAGGGTACCGTTAACGCTCCCCGAAAACGGC | Promoter PBMEII0192 |
| Promoter-R | AGTCGACATATGTGATTTCCGGC | |
| Flag-F | ACATATGAGCCTAGTCGACTACAAAGACCATGAC | pBBR1Flag |
| Flag-R | AGAGCTCCTGCAGTTACTATTTATCGTCGTCATC | |
| A0598-Flag-F | ACATATGAGAGCAACCACGCTTAC | pCydX/Flag |
| A0598 -PstI | ACTGCAGTCAGTGGTGCTTGTTGCCTTC |
*
Engineered restriction sites underlined.