Table 1.
Distribution of reads aligned to the human reference genome: average (standard deviation) in millions, and percentage
| Overall | Inside TR | Outside ≤ 200 bp | Outside >200 bp | ||||
|---|---|---|---|---|---|---|---|
| SureSelect (n = 22) |
# Reads |
# Reads |
% |
# Reads |
% |
# Reads |
% |
| Before filter |
63.4 (9.6) |
34.3 (4.9) |
54.1 |
6.4 (1.2) |
10.1 |
22.7 (3.9) |
35.8 |
| After filter |
57.2 (8.6) |
32.0 (4.5) |
56.1 |
6.0 (1.1) |
10.4 |
19.2 (3.3) |
33.5 |
| TrueSeq (n = 6) | |||||||
| Before filter |
86.2 (2.5) |
37.0 (0.7) |
42.9 |
17.1 (0.3) |
19.8 |
32.1 (1.3) |
37.2 |
| After filter |
75.1 (2.1) |
34.8 (0.7) |
46.3 |
16.3 (0.3) |
21.7 |
24.0 (1.1) |
32.0 |
| 1000 G (n = 6) | |||||||
| Before filter |
33.9 (8.1) |
7.6 (1.2) |
22.3 |
4.7 (1.7) |
14.0 |
21.6 (8.4) |
63.7 |
| After filter | 25.6 (3.8) | 7.3 (1.1) | 28.7 | 4.7 (1.7) | 18.6 | 13.6 (3.6) | 52.7 |
The filter was MAPQ ≥ 20.