Fig 2.
Quantification of Ata expression in A. baumannii ATCC 17978 by flow cytometry during various growth phases. A. baumannii was grown in LB to early exponential (OD650 of 0.025 or 0.1), mid-exponential (OD650 of 0.4), late exponential (OD650 of 0.8), or stationary phase (OD650 of 1.2), labeled with rabbit antibody to Ata and secondary goat antibody to rabbit IgG conjugated to Alexa 488 fluorescent dye, and analyzed by flow cytometry. Results represent the mean fluorescent intensity (MFI) of 500,000 cells, and bars indicate the averages from three independent experiments ± standard errors of the means (SEM).