Skip to main content
. Author manuscript; available in PMC: 2013 Aug 3.
Published in final edited form as: Cell. 2012 Aug 3;150(3):620–632. doi: 10.1016/j.cell.2012.06.027

Figure 2. SirT1 mimics Pparγ ligand in regulating adipocyte gene expression.

Figure 2

(A) Expression of white genes in 3T3-L1 adipocytes treated with 50 μM resveratrol or vehicle (Veh) overnight on differentiation day 7. *: P<0.05, **: P<0.01 vs. vehicle (n=3).

(B) White gene expression in 3T3-L1 adipocytes overexpressing GFP, SirT1 or Sirt1-H363Y (HY). Fully differentiated cells were harvested on day 8 of differentiation. *: P<0.05, **: P<0.01 vs. GFP (n=7).

(C) Ucp1 expression in HIB-1B brown adipocytes with treatments overnight on day 6 of differentiation. **: P<0.01 vs. vehicle-treated controls (n=3-4).

(D-E) Protein (D) and gene expression analysis (E) in HIB-1B cells overexpressing GFP, SirT1 and Sirt1-H363Y (HY). IP: immunoprecipitation, IB: immunoblot; *: P<0.05, **: P<0.01 vs. GFP (n=3-9). Values are presented as means ± SEM.

See also Figure S2.