Figure 1.
Protective effect of Nec-1 and HNG on oxygen-glucose deprivation (OGD)-induced cell death in primary cortical neurons. Primary cortical neurons were used to perform OGD at DIV 10. Neurons were washed with HBSS and incubated with vehicle, Nec-1 (25 μM), HNG (0.2 μM), or both in a hypoxia chamber for 60 min. Cell viability was determined by MTS assay 24 h after reperfusion. Control neurons were exposed to oxygenated HBSS containing 5.5 mM glucose in normoxic conditions during the same time as the OGD culture. Bars represent mean ± SEM of 8 samples. *P<0.01, versus OGD group; #, P<0.01, versus OGD+HNG+Nec-1 group.