Figure 2. Lipidomics platform.
Lipid extracts (dark blue) enter a workflow involving a universal normal phase HPLC-MS system (black), software-assisted raw data extraction (lime green), computational comparative analysis (red), database and dataset annotation (purple), and molecular discovery through collisional mass spectrometry (light blue). This second generation system for comparative profiling emphasizes a single-step chromatography system, in contrast to a first generation method that uses fluid phase separation and multiple HPLC systems (Figure S2). (B) Extracted ion chromatograms of the overall features detected with high, intermediate and low intensity by analyzing M. tuberculosis H37Rv total lipids.