Fig. 2.
Conversion of astrocytes into NSCs by OCT4/SOX2 and NANOG does not require the pluripotent state.
a) A colony of CD44 + NANOG-NSCs presented a different morphological aspect under contrast-phase microscopic observation relative to the iPSC colony obtained from fibroblasts by contemporaneous transfection of OCT4/SOX2/NANOG/LIN28. iPSCs were positive for pluripotency markers SSEA3 and TRA-1-60. Scale bar: 150 μm. b) Quantitative analysis of SSEA3 and Tra-1-60 in Astro, AstroNANOG, and human fibroblasts transduced with OCT4, SOX2, NANOG, and LIN28, over the human iPSC derivation timeline. c) Pluripotency gene profile in parental astrocytes and in AstroNANOG. d) Teratomas derived from human iPSCs and testicular sections from mice injected with astrocytes, AstroNANOG, and saline (CTR). Scale bar: 300 μm.