Figure 2.
Cluvenone competes with GA-Bodipy for sites in mitochondria. HeLa cells were incubated with MitoTracker Red at 50 nM for 15 min and then fixed. Fixed cells were then treated with 1 μM GA-Bodipy for 30 min followed by washing. Cells were then further incubated with 0.1% DMSO (a, b, c) or 20μM cluvenone (d, e, f) for 1h followed by washing. Fluorescence microscopy was performed with excitation at 579 nm and emission at 599 nm. Mitochondria (red) are shown in c) and f). BODIPY fluorescence (green) is shown in b) and e). Merged images are in a) and d).