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. Author manuscript; available in PMC: 2012 Jul 25.
Published in final edited form as: J Immunol. 2011 Apr 11;186(10):5938–5948. doi: 10.4049/jimmunol.1002635

Figure 6. PVM-induced upregulation of IFN–α expression by BM-derived pDC requires TLR7.

Figure 6

(A) Bone marrow cells were harvested and cultured in Flt3L to generate a pDC-rich cell population (~20%). After a 10 day culture, cells were cultured overnight with PVM at an MOI of 1 in the presence of brefeldin A. IFN–α was detected by intracellular cytokine staining and localised to Siglec-H+, B220+ cells. (B) The percentage of Siglec+IFN–α+ cells as a fraction of total Flt3L-treated WT and TLR7−/− bone marrow cells cultured with or without PVM. Data are mean +/− s.e.m, 3–4 mice in each group and ** p<0.01.