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. 2012 May 2;4(7):660–673. doi: 10.1002/emmm.201200243

Figure 7. Increased nitrotyrosination of γ-secretase in the brain of 18 months SOD2+/− mice correlates with a switch in its activity towards a higher Aβ42 production.

Figure 7

  1. Brains of 18 months Sod2+/− mice (a and b) show consistently higher nitrotyrosination signal (green) in the parenchyma compared to age-matched wt mice (c and d). The nucleus of the cells are stained with DAPI (blue) in (e) and (f) only secondary antibodies were used. The hippocampal area is shown.
  2. PS1-CTF was inmunoprecipitated from 0.5% Triton-X-100 solubilized extracts (f) the cerebral cortex of 18 months SOD2+/− mice. More PS1-NTF was co-immuneprecipitated as demonstrated by Western blot in SOD+/− compared to SOD+/+ (wt) controls (**p < 0.01, n = 6).
  3. Cell-free γ-secretase activity using microsomal extracts from the cerebral cortex of 18 months SOD2+/− or wt mice. 3xFlag-huAPP-C99 was used as a substrate at 0.5 µM. The Aβ42/Aβ40 ratio is increased (*p < 0.05, n = 6). Data are presented as mean ± SEM.