Figure 5.
XCR1+ DCs specifically take up cell-associated antigen. C57BL/6 mice were injected with 10 × 106 CFSE-labeled allogeneic 300-19-ΔOVA cells i.v., and cDCs (CD11c+MHCII+Lin− cells) were analyzed for CFSE signals 2, 4, 12, and 24 h later. (A) The XCR1+CD8+ (red), XCR1+CD8− (only here black!), XCR1−CD8+ (green), and XCR1−CD8− (blue) subpopulations are color-coded. Note that XCR+CD8− DCs express lower levels of XCR1 compared to XCR1+CD8+ DCs. Shown is one representative experiment out of three. (B) Relative ratio of cell uptake capacity, calculated for each animal by setting the frequency of XCR1−CD8− DCs exhibiting a CFSE-signal to 1 and calculating the fold higher uptake for the other DC subsets accordingly (mean ± SEM of two independent experiments with three animals each; *p < 0.05; **p < 0.01; ***p < 0.001).