Figure 5.
Knockdown of IL-6R induces cell death in cerebral cortical neurons, and enhancement of IL-6R by IL-6 reduces infarct volumes in cerebral ischemic injury. A, Cell death assessed by LDH activity in a medium of cortical neurons transfected with IL-6Rα- or gp130-specific siRNA for 24 hours or treated with AG490 for 24 hours (n=4 per group). Data are mean± S.E.M. *P<0.05, **P<0.001 vs. control. B, LDH activity in a medium of cortical neurons transfected with IL-6Rα- or gp130-specific siRNA for 24 hours in SOD2 WT or SOD2-/+ KO mouse brains (n=4 per group). Data are mean ± S.E.M. *P<0.05 vs. control. C, 2,3,5-triphenyltetrazolium chloride staining for analysis of infarct volumes after 24 hours of reperfusion following MCAO with or without IL-6 injection in male mice. D, Summary graph showing the size of infarction volumes. *P<0.05 (n=9 per group). NS indicates not significant; O.D., optical density.