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. Author manuscript; available in PMC: 2013 Jun 8.
Published in final edited form as: Circ Res. 2012 Apr 24;110(12):1604–1617. doi: 10.1161/CIRCRESAHA.111.261768

Figure 7. Stimulation of the AT1A receptor on T lymphocytes constrains Th1 differentiation in the setting of hypertension.

Figure 7

Figure 7

Figure 7

Figure 7

CD4+ T cells were isolated via fluorescent cell sorting from the spleens of Ang II-infused T cell WT and KO animals and characterized for Th1 vs. Th2 phenotype. A, mRNA expressions of IFN-γ, TNF-α, IL-4, and TGF-β in CD4+ T lymphocytes from T cell WT and KO mice (n≥6). *P<0.03 vs. T cell WT; †P=0.03 vs. T cell WT. B, concentrations of IFN-γ, TNF-α, and IL-4 proteins in supernatants of activated splenic lymphocytes from Ang II-infused T cell WT and KO mice (n=6). *P=0.05 vs. T cell WT; #P<0.005 vs. T cell WT. C, ratio of mRNA expression of T-bet/GATA3 in purified CD4+ T lymphocytes from T cell WT and KO mice (n=5–6). *P=0.05 vs. Ang II T cell WT. D, co-expression of PD-1 and TIM-3 measured by flow cytometry is similar on splenic CD4+ T cells from T cell WT and KO mice (n≥6). Representative dotplots are shown. Summary data are presented in the text.